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Q&A ViewAttendees 10

Physiology, Biochemistry, and Toxicology

10-Minute Paper

PBT: Biochemistry and Entomophagy

Linking transcriptomics, proteomics and lipidomics in the fat body of the Colorado potato beetle: Highlights of lipid metabolism in relation to diapause

Monday, November 1, 2021
1:54 PM – 2:06 PM MT
Location: Colorado Convention Center, Meeting Room 403-404
In-Person

    Presenting Author(s)

  • Umut Toprak

    Associate Professor
    Ankara University
    Ankara, Ankara, Turkey

    • Co-Author(s)

  • B

    Şerife Bayram

    Emeritus professor
    Ankara University
    Ankara, Ankara, Turkey

  • DB

    Doug Baldwin

    Research Technician
    Saskatoon Research Center
    Saskatoon, Saskatchewan, Canada

  • CC

    Cathy Coutu

    Saskatoon Research Center
    Saskatoon, Saskatchewan, Canada

  • DH

    Dwayne Hegedus

    Agriculture and Agri-Food Canada, Canada

  • RG

    Russell Groves

    University of Wisconsin
    Madison, Wisconsin

  • GG

    Gözde Güney

    Ankara University
    Ankara, Ankara, Turkey

  • Cansu Doğan

    Graduate Research Assistant
    Ankara University
    Ankara, Ankara, Turkey

  • DC

    Doğa Cedden

    Ankara University
    Ankara, Ankara, Turkey

  • DH

    David Heckel

    Max Planck Institute for Chemical Ecology
    Jena, Thuringen, Germany

The Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), is the major defoliator of potato in the northern hemisphere. Adults overwinter under soil around 7 months of their lives in hibernation diapause and emerge from the soil by spring. Successful maintenance of diapause and emergence is closely related with stored lipids before diapause and their hydrolysis that occur in the fat body. In the current study, we followed multiple “omics” approaches; transcriptomics, proteomic and lipidomics of the fat body from actively-feeding larvae and adults, hibernating adults, and adults emerged from hibernation. 4,051 of a total of 30,842 transcripts revealed significant differential gene expression between the stages, while proteomic analyses generated a total of 1,621 proteins from 1,471 different clusters. Amongst these, 806, 410, 402 and 1,220 proteins were found to be associated with the fat body from feeding larvae, feeding adults, hibernating adults and adults emerged from hibernation, respectively. Lipidomic analysis revealed presence of 247 and 218 lipids in positive and negative modes, respectively. More specifically, 47 and 28.7% of the total lipids were triglycerides and fatty acids in the positive mode. The most predominant lipids in the stages together with the potential associated transcripts and proteins are presented within a model, which provides key clues in understanding the lipid metabolism and how they affect the phases of diapause in L. decemlineata. This research was supported by TÜBİTAK (Grant #:118O976).