Plant-Insect Ecosystems
10-Minute Paper
Daniel Kunk
PhD Student
Colorado State University
Fort Collins, Colorado
Circadian rhythms of insect pests and their host plants influence the outcome of their interactions. However, knowledge of these interactions in agriculturally important crops and their pests is lacking. Here we sought to understand how the circadian clock in aphids (Rhopalosiphum padi L.) influences their performance and feeding behavior on bread wheat (Triticum aestivum L. cv. Chinese Spring). We determined the feeding behaviors of R. padi on wheat using the electrical penetration graph technique (EPG). The influence of diurnal rhythms on plants as determined by comparing aphid behaviors on plants and aphids entrained on a 12 h light/dark cycle vs. plants and aphid entrained to a constant light regime. In a second experiment, the influence of the host diurnal rhythms was eliminated by determining R. padi feeding behavior on artificial diet in light/dark and constant light conditions. The outcomes of this experiment represent a first step towards understanding the role of the aphid’s circadian clock in regulating feeding behaviors.