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Systematics, Evolution, and Biodiversity

Member Symposium

Tephritidae Systematics, Diagnostics, Phylogeny, Evolution & Natural History

A new phylogeny for Anastrepha (Diptera: Tephritidae) from AHE and the information content in nuclear loci for species identification

Tuesday, November 2, 2021
3:50 PM – 4:05 PM MT
Location: Colorado Convention Center, Meeting Room 205-207
In-Person
With over 300 named species, Anastrepha is the largest group of fruit flies within Latin America and the Caribbean. They include the most economically important pest species in the region, some that have invaded the US and others that remain serious threats to U.S. agriculture. We are using phylogenomic methods and nextgen sequencing of nuclear protein encoding loci from over 100 species to reconstruct phylogenetic relationships with the genus. These genomic data are also used to evaluate nuclear loci as markers in PCR-based rapid identification for diagnosing pest species within this genus. Data are presented from application of the anchored hybrid enrichment (AHE) techniques resulting in over 700 loci that yield valuable evidence on the phylogeny of the genus. AHE generated gene loci are also used to amplify diagnostic markers that accurately identify known and potential pest-, species. We also report on additional work that examines the utility of sequencing mitochondrial genomes for revealing informative loci. The results of diagnostic assay validation tests are presented for comparison of nuclear gene data vs. mitochondrial data in evaluation of species identity. An identification system and on-line, interactive phylogeny tool, are demonstrated to facilitate Anastrepha species identification.